Latitudinal Characteristic Nodule Composition of Soybean-Nodulating Bradyrhizobia: Temperature-Dependent Proliferation in Soil or Infection?

A species-specific latitudinal distribution of soybean rhizobia has been reported; Bradyrhizobium japonicum and B. elkanii dominate in nodules northern southern areas, respectively. The aim this study was to elucidate whether temperature-dependent proliferation soil or infection is more reliable for determining the characteristic soybean-nodulating under local climate conditions. Three locations, Fukagawa (temperate continental climate), Matsue Miyazaki (humid sub-tropical were selected Japan. Each sample transported other cv. Orihime (non-Rj) pot-cultivated using three soils at locations two successive years. Species composition analyzed based on partial 16S rRNA 16S–23S ITS gene sequences. Two (Bj11 BjS10J) clusters one (BeL7) cluster phylogenetically sub-grouped into (Bj11-1-2) four (BjS10J-1-4) sequence. In soil, Bj11-1 dominated (80–87%) all locations. similar which BeL7 (70–73%), while location, decreased 53% BjS10J-3 increased. 77%, 13% 33% respectively, BjS10J-2 BjS10J-4 It supposed that strain preferably proliferated leading its nodule dominancy, would lead dominancy elkanii, both factors be involved location.